...catalyzes DNA integration of viral DNA into the host-cell genome.
Human immunodeficiency virus type 1 (HIV-1), like all retroviruses, depends upon the integration of a DNA copy of its viral genome into host cell chromosomes as part of its infection cycle. The HIV-1 integrase (IN) enzyme mediates this insertion of viral DNA into the human genome and it is one of three virally-encoded enzymes required for virus replication. Because the proviral integration event is essential for HIV-1 replication, the HIV-1 IN enzyme remains a promising target for antiviral drug development to treat patients with AIDS.
This purified HIV-1 Integrase prepartion is enzymatically active in DNA strand-transfer assays.
SOURCE: |
HIV-1 Integrase is an affinity-purified enzyme that catalyzes the insertion of proviral DNA sequences into target DNA. This enzyme contains the amino acid substitutions F185H. Like the F185K mutation (J Biol Chem 271:7712, 1996), the F185H mutation makes the enzyme more soluble without affecting in vitro activity. |
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| RECONSTITUTION: |
Reconstitute lyophilized HIV-1 Integrase in 100 µL deionized H2O for 100 µg quantity. The enzyme is stable to freeze-thawing. | |
| CONCENTRATION: |
1 mg/mL in deionized H2O after reconstitution. | |
| PURITY & STERILITY: |
HIV-1 Integrase has been shown to be > 90% pure by SDS-PAGE. | |
| STORAGE & HANDLING: |
Store lyophilized sample at -20C or -70C prior to reconstitution. Once the integrase enzyme has been reconstituted, store at -70C. |
ORDERING INFORMATION:
